RT Journal Article
SR Electronic
T1 The endoplasmic reticulum membrane protein complex (EMC) negatively regulates intestinal homeostasis through the Hippo signaling pathway
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.01.25.477727
DO 10.1101/2022.01.25.477727
A1 Lin Shi
A1 Hubing Ma
A1 Hang Zhao
A1 Meifang Ma
A1 Jinjun Wang
A1 Ruiyan Kong
A1 Zhengran Li
A1 Rui Ma
A1 Jian-Hua Wang
A1 Shian Wu
A1 Meng-qiu Dong
A1 Zhouhua Li
YR 2022
UL http://biorxiv.org/content/early/2022/01/26/2022.01.25.477727.abstract
AB Disruption of tissue homeostasis often results in many diseases. Balanced control of stem cell proliferation and differentiation underlines tissue homeostasis. However, how endogenous factors influence the proliferation and differentiation of intestinal stem cells (ISCs) under physiological conditions remains not fully understood. Here, we find that the endoplasmic reticulum membrane protein complex (EMC) negatively regulates ISC proliferation in adult Drosophila midgut. Compromising EMC function in progenitors leads to excessive ISC proliferation and intestinal homeostasis disruption. Mechanistically, the EMC complex associates with and stabilizes Hippo (Hpo), the key component of the Hpo signaling pathway. In the absence of the EMC complex, Yki (Yorkie) is activated to promote ISC proliferation. Furthermore, the role of the EMC complex in stem cell proliferation control is evolutionarily conserved. Thus, our study uncovers the molecular mechanism of the EMC complex in controlling stem cell proliferation. Our results provide new insight into the underlying mechanisms of how stem cell proliferation is properly controlled under physiological conditions.Competing Interest StatementThe authors have declared no competing interest.