PT  - JOURNAL ARTICLE
AU  - Lilya Andrianova
AU  - Steliana Yanakieva
AU  - Gabriella Margetts-Smith
AU  - Shivali Kohli
AU  - Erica S Brady
AU  - John P Aggleton
AU  - Michael T Craig
TI  - No evidence from complementary data sources of a direct projection from the mouse anterior cingulate cortex to the hippocampal formation
AID  - 10.1101/2022.01.25.477805
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.25.477805
4099  - http://biorxiv.org/content/early/2022/01/26/2022.01.25.477805.short
4100  - http://biorxiv.org/content/early/2022/01/26/2022.01.25.477805.full
AB  - The connectivity and interplay between the prefrontal cortex and hippocampus underpin a number of key cognitive processes, with changes in these interactions being implicated in both neurodevelopmental as well as neurodegenerative conditions. Understanding the precise cellular connections through which this circuit is organised is, therefore, vital for understanding these same processes. Overturning earlier findings, a recent study described a novel excitatory projection from anterior cingulate cortex to hippocampus. We sought to validate this unexpected finding using multiple, complementary methods: anterograde and retrograde anatomical tracing, using both anterograde and retrograde AAVs and monosynaptic rabies tracing. Additionally, an extensive data search of the Allen Projection Brain Atlas database was conducted to find the stated projection within any of the deposited anatomical studies, as an independent verification of our own results. However, we failed to find any evidence of a direct, monosynaptic projection from mouse anterior cingulate cortex to the hippocampus proper.Competing Interest StatementThe authors have declared no competing interest.