TY - JOUR T1 - Sen1 is required for RNA Polymerase III transcription termination in an R-loop independent manner JF - bioRxiv DO - 10.1101/571737 SP - 571737 AU - Julieta Rivosecchi AU - Marc Larochelle AU - Camille Teste AU - Frédéric Grenier AU - Amélie Malapert AU - Emiliano P. Ricci AU - Pascal Bernard AU - François Bachand AU - Vincent Vanoosthuyse Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/03/11/571737.abstract N2 - R-loop disassembly by the human helicase Senataxin contributes to genome stability and to proper transcription termination at a subset of RNA polymerase II genes. Whether Senataxin-mediated R-loop disassembly also contributes to transcription termination at other classes of genes has remained unclear. Here we show in fission yeast that SenataxinSen1 promotes efficient termination of RNA Polymerase III (RNAP3) transcription in vivo. In the absence of SenataxinSen1, RNAP3 accumulates downstream of the primary terminator at RNAP3-transcribed genes and produces long exosome-sensitive 3’-extended transcripts. Importantly, neither of these defects was affected by the removal of R-loops. The finding that SenataxinSen1 acts as an ancillary factor for RNAP3 transcription termination in vivo challenges the pre-existing view that RNAP3 terminates transcription autonomously. We propose that Senataxin is a cofactor for transcription termination that has been co-opted by different RNA polymerases in the course of evolution. ER -