TY - JOUR T1 - Dynamic observation of <sup>2</sup>H labeled compounds in the human brain with <sup>1</sup>H versus <sup>2</sup>H magnetic resonance spectroscopy at 9.4T JF - bioRxiv DO - 10.1101/2022.01.24.477582 SP - 2022.01.24.477582 AU - Loreen Ruhm AU - Theresia Ziegs AU - Andrew Martin Wright AU - Claudius Sebastian Mathy AU - Saipavitra Murali-Manohar AU - Johanna Dorst AU - Nikolai Avdievich AU - Anke Henning Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/01/27/2022.01.24.477582.abstract N2 - The metabolic pathway of [6,6’-2H2]-labeled glucose was investigated with two different techniques. The first technique used direct detection of deuterium applying Deuterium Metabolic Imaging (DMI). The second technique used the indirect detection of deuterium with proton MR spectroscopy (MRS) called Quantitative Exchanged-label Turnover (QELT) MRS. For the first time, time-resolved data was acquired for both techniques in the same healthy human subjects and directly compared. The time-curves were used in a kinetic model to estimate rates of the metabolic pathway of glucose. Two different kinetic models were compared. One included only DMI data, the second one combined DMI and QELT. For the first model, a tricarboxylic acid (TCA) cycle rate of 0.69 ± 0.10 μmol·min-1·g-1 was determined. For the second model, the estimated TCA cycle rate was 0.68 ± 0.12 μmol·min-1·g-1. In addition, the rate of glutamine synthesis from glutamate could be estimated with model 2 (0.51 ± 0.15 μmol·min-1·g-1). The sensitivity of both methods was evaluated and compared to alternative techniques.Competing Interest StatementThe authors have declared no competing interest. ER -