RT Journal Article
SR Electronic
T1 A catalogue of resistance gene homologs and a chromosome-scale reference sequence support resistance gene mapping in winter wheat
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.01.26.477808
DO 10.1101/2022.01.26.477808
A1 Sandip M. Kale
A1 Albert W. Schulthess
A1 Sudharsan Padmarasu
A1 Philipp H. G. Boeven
A1 Johannes Schacht
A1 Axel Himmelbach
A1 Burkhard Steuernagel
A1 Brande B. H. Wulff
A1 Jochen C. Reif
A1 Nils Stein
A1 Martin Mascher
YR 2022
UL http://biorxiv.org/content/early/2022/01/28/2022.01.26.477808.abstract
AB A resistance gene atlas is an integral component of the breeder’s arsenal in the fight against evolving pathogens. Thanks to high-throughput sequencing, catalogues of resistance genes can be assembled even in crop species with large and polyploid genomes. Here, we report on capture sequencing and assembly of resistance gene homologs in a diversity panel of 907 winter wheat genotypes comprising ex situ genebank accessions and current elite cultivars. In addition, we use accurate long-read sequencing and chromosome conformation capture sequencing to construct a chromosome-scale genome sequence assembly of cv. Attraktion, an elite variety representative of European winter wheat. We illustrate the value of our resource for breeders and geneticists by (i) comparing the resistance gene complements in plant genetic resources and elite varieties and (ii) conducting genome-wide associations scans (GWAS) for the fungal diseases yellow rust and leaf rust using reference-based and reference-free GWAS approaches. The gene content under GWAS peaks was scrutinized in the assembly of cv. Attraktion.Competing Interest StatementThe authors have declared no competing interest.