RT Journal Article
SR Electronic
T1 LuminoCell: a versatile and affordable luminometer platform for monitoring in-cell luciferase-based reporters
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.01.28.478134
DO 10.1101/2022.01.28.478134
A1 Kamila Weissová
A1 Bohumil Fafílek
A1 Tomasz Radaszkiewicz
A1 Canan Celiker
A1 Petra Macháčková
A1 Tamara Čechová
A1 Jana Šebestíková
A1 Aleš Hampl
A1 Vítězslav Bryja
A1 Pavel Krejčí
A1 Tomáš Bárta
YR 2022
UL http://biorxiv.org/content/early/2022/01/28/2022.01.28.478134.abstract
AB Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility of their use in different experimental conditions. Therefore, we aimed to develop a small, affordable luminometer allowing continuous measurement of luciferase activity, designed for inclusion into various kinds of tissue culture incubators. Here we introduce LuminoCell - an open-source platform for the construction of an affordable, sensitive, and portable luminometer capable of realtime monitoring in-cell luciferase activity. The LuminoCell costs $40, requires less than 1 hour to assemble, and it is capable of performing real-time sensitive detection of both magnitude and duration of the activity of major signalling pathways in cell cultures, including receptor tyrosine kinases (EGF, FGF), WNT/β-catenin, and NF-κB. Additionally, we show that the LuminoCell is suitable to be used in cytotoxicity assays as well as for monitoring periodic circadian gene expression.Competing Interest StatementThe authors have declared no competing interest.