PT  - JOURNAL ARTICLE
AU  - Yuri S. van Ekelenburg
AU  - Karina S. Hornslien
AU  - Tom Van Hautegem
AU  - Matyáš Fendrych
AU  - Gert Van Isterdael
AU  - Katrine N. Bjerkan
AU  - Jason R. Miller
AU  - Moritz K. Nowack
AU  - Paul E. Grini
TI  - Spatial and Temporal Regulation of Parent-of-Origin Allelic Expression in the Endosperm
AID  - 10.1101/2022.01.29.478178
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.29.478178
4099  - http://biorxiv.org/content/early/2022/01/30/2022.01.29.478178.short
4100  - http://biorxiv.org/content/early/2022/01/30/2022.01.29.478178.full
AB  - Genomic imprinting promotes differential expression of parental alleles in the endosperm of flowering plants, and is regulated by epigenetic modification such as DNA methylation and histone tail modifications in chromatin. After fertilization, the endosperm develops through a syncytial stage before it cellularizes and becomes a nutrient source for the growing embryo. Both in early and late endosperm development regional compartmentalization has been shown, and different transcriptional domains suggest divergent spatial and temporal regional functions. The analysis of the role of parent-of-origin allelic expression in the endosperm as a whole and also investigation of domain specific functions has been hampered by the availability of the tissue for high-throughput transcriptome analyses and contamination from surrounding tissue. Here we have used Fluorescence-Activated Nuclear Sorting (FANS) of nuclear targeted eGFP fluorescent genetic markers to capture parental specific allelic expression from different developmental stages and specific endosperm domains. This RNASeq approach allows us to successfully identify differential genomic imprinting with temporal and spatial resolution. In a systematic approach we report temporal regulation of imprinted genes in the endosperm as well as region specific imprinting in endosperm domains. Our data identifies loci that are spatially differentially imprinted in one domain of the endosperm while biparentally expressed in other domains. This suggests that regulation of genomic imprinting is dynamic and challenges the canonical mechanisms for genomic imprinting.Competing Interest StatementThe authors have declared no competing interest.