TY - JOUR T1 - Using the Volta phase plate with defocus for cryo-EM single particle analysis JF - bioRxiv DO - 10.1101/085530 SP - 085530 AU - Radostin Danev AU - Dimitry Tegunov AU - Wolfgang Baumeister Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/11/03/085530.abstract N2 - Previously, we reported an in-focus data acquisition method for cryo-EM single particle analysis with the Volta phase plate (VPP) (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also enables 3D reconstructions with resolutions in the 2 Å range, demonstrating that, in practice, there are no resolution limitations imposed by the VPP. ER -