PT  - JOURNAL ARTICLE
AU  - Sheldon J.J. Kwok
AU  - Daniel T. Montoro
AU  - Adam L. Haber
AU  - Seok-Hyun Yun
AU  - Vladimir Vinarsky
TI  - Single-cell transcriptomics of dynamic cell behaviors
AID  - 10.1101/2022.01.31.478519
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.31.478519
4099  - http://biorxiv.org/content/early/2022/01/31/2022.01.31.478519.short
4100  - http://biorxiv.org/content/early/2022/01/31/2022.01.31.478519.full
AB  - Despite advances in spatial transcriptomics, the molecular profiling of dynamic behaviors of cells in their native environment remains a major challenge. We present a method, termed behavioral transcriptomics, that allows us to couple physiological behaviors of single cells in an intact tissue to deep molecular profiling of individual cells. This method enabled us to establish a novel molecular signature for a striking migratory cellular behavior following tissue injury.Competing Interest StatementSJJK is currently an employee of and has financial interests in LASE Innovation Inc. SHY has financial interests in LASE Innovation Inc. that were reviewed and are managed by Massachusetts General Hospital and Mass General Brigham in accordance with their conflict-of-interest policies. VV is currently an employee and has financial interest in Vertex Pharmaceuticals, Inc. All other authors declare they have no competing interests.