TY - JOUR T1 - Sentinel Cards Provide Practical SARS-CoV-2 Monitoring in School Settings JF - bioRxiv DO - 10.1101/2022.02.01.478759 SP - 2022.02.01.478759 AU - Victor J Cantú AU - Karenina Sanders AU - Pedro Belda-Ferre AU - Rodolfo A Salido AU - Rebecca Tsai AU - Brett Austin AU - William Jordan AU - Menka Asudani AU - Amanda Walster AU - Celestine G. Magallanes AU - Holly Valentine AU - Araz Manjoonian AU - Carrissa Wijaya AU - Vinton Omaleki AU - Stefan Aigner AU - Nathan A Baer AU - Maryann Betty AU - Anelizze Castro-Martínez AU - Willi Cheung AU - Peter De Hoff AU - Emily Eisner AU - Abbas Hakim AU - Alma L Lastrella AU - Elijah S Lawrence AU - Toan T Ngo AU - Tyler Ostrander AU - Ashley Plascencia AU - Shashank Sathe AU - Elizabeth W Smoot AU - Aaron F Carlin AU - Gene W Yeo AU - Louise C Laurent AU - Anna Liza Manlutac AU - Rebecca Fielding-Miller AU - Rob Knight Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/02/03/2022.02.01.478759.abstract N2 - Accurate, high-resolution environmental monitoring of SARS-CoV-2 traces indoors through sentinel cards is a promising approach to help students safely return to in-person learning. Because SARS-CoV-2 RNA can persist for up to a week on several indoor surface types, there is a need for increased temporal resolution to determine whether consecutive surface positives arise from new infection events or continue to report past events. Cleaning sentinel cards after sampling would provide the needed resolution, but might interfere with assay performance. We tested the effect of three cleaning solutions (BZK wipes, wet wipes, RNase Away) at three different viral loads: “high” (4 x 104 GE/mL), “medium” (1 x 104 GE/mL), and “low” (2.5 x 103 GE/mL). RNAse Away, chosen as a positive control, was the most effective cleaning solution on all three viral loads. Wet wipes were found to be more effective than BZK wipes in the medium viral load condition. The low viral load condition was easily reset with all three cleaning solutions. These findings will enable temporal SARS-CoV-2 monitoring in indoor environments where transmission risk of the virus is high and the need to avoid individual-level sampling for privacy or compliance reasons exists.Importance Because SARS-CoV-2, the virus that causes COVID-19, persists on surfaces, testing swabs taken from surfaces is useful as a monitoring tool. This approach is especially valuable in school settings, where there are cost and privacy concerns that are eliminated by taking a single sample from a classroom. However, the virus persists for days to weeks on surface samples, so it is impossible to tell whether positive detection events on consecutive days are persistent signal or new infectious cases, and therefore whether the positive individuals have been successfully removed from the classroom. We compare several methods for cleaning “sentinel cards” to show that this approach can be used to identify new SARS-CoV-2 signals day to day. The results are important for determining how to monitor classrooms and other indoor environments for SARS-CoV-2 virus. ER -