RT Journal Article
SR Electronic
T1 Single cell transcriptomic and spatial landscapes of the developing human pancreas
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.02.04.478971
DO 10.1101/2022.02.04.478971
A1 Oladapo E. Olaniru
A1 Ulrich Kadolsky
A1 Shichina Kannambath
A1 Heli Vaikkinen
A1 Kathy Fung
A1 Pawan Dhami
A1 Shanta J. Persaud
YR 2022
UL http://biorxiv.org/content/early/2022/02/04/2022.02.04.478971.abstract
AB The progress made in directed differentiation of stem cells has shown that understanding human pancreas development can provide cues for generating unlimited amounts of insulin-producing beta cells for transplantation therapy in diabetes. However, current differentiation protocols have not been successful in reproducibly generating functional human beta cells in vitro, partly due to incomplete understanding of human pancreas development. Here, we present detailed transcriptomic analysis of the various cell types of the developing human pancreas, including their spatial gene patterns. We integrated single cell RNA sequencing with spatial transcriptomics at multiple developmental timepoints and revealed distinct temporal-spatial gene cascades in the developing human pancreas. Cell trajectory inference identified endocrine progenitor populations and novel branch-specific genes as the progenitors differentiate towards alpha or beta cells, indicating that transcriptional maturation occurred over this developmental timeframe. Spatial differentiation trajectories indicated that immature Schwann cells are spatially co-located with endocrine progenitors and contribute to beta cell maturation via the L1CAM-EPHB2 pathway. Our integrated approach enabled us to identify heterogeneity and multiple lineage dynamics within the mesenchyme, showing that it contributed to the exocrine acinar cell state. Finally, we have generated an interactive web resource for interrogating human pancreas development for the research community.Competing Interest StatementThe authors have declared no competing interest.