TY - JOUR T1 - Novel cerebello-amygdala connections provide missing link between cerebellum and limbic system JF - bioRxiv DO - 10.1101/2022.02.07.479043 SP - 2022.02.07.479043 AU - Se Jung Jung AU - Ksenia Vlasov AU - Alexa D’Ambra AU - Abhijna Parigi AU - Mihir Baya AU - Edbertt Paul Frez AU - Jacqueline Villalobos AU - Marina Fernandez-Frentzel AU - Maribel Anguiano AU - Yoichiro Ideguchi AU - Evan G. Antzoulatos AU - Diasynou Fioravante Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/02/07/2022.02.07.479043.abstract N2 - The cerebellum is emerging as a powerful regulator of cognitive and affective processing and memory in both humans and animals and has been implicated in affective disorders. How the cerebellum supports affective function remains poorly understood. The short-latency (just a few ms) functional connections that were identified between the cerebellum and amygdala -a structure crucial for the processing of emotion and valence-more than 4 decades ago raise the exciting, yet untested, possibility that a cerebellum-amygdala pathway communicates information important for emotion. The major hurdle in rigorously testing this possibility is the lack of knowledge about the anatomy and functional connectivity of this pathway. Our initial anatomical tracing studies in mice excluded the existence of a direct monosynaptic pathway between cerebellum and amygdala. Using transneuronal tracing techniques, we have identified a novel disynaptic pathway that connects the cerebellar output nuclei to the basolateral amygdala. This pathway recruits the understudied intralaminar thalamus as a node. Using ex vivo optophysiology and super-resolution microscopy, we provide the first evidence for the functionality of the pathway, thus offering a missing mechanistic link between the cerebellum and amygdala. This discovery provides a connectivity blueprint between the cerebellum and a key structure of the limbic system. As such, it is the requisite first step toward obtaining new knowledge about cerebellar function in emotion, thus fundamentally advancing understanding of the neurobiology of emotion, which is perturbed in mental and autism spectrum disorders.Competing Interest StatementThe authors have declared no competing interest. ER -