PT  - JOURNAL ARTICLE
AU  - Gen Xu
AU  - Jing Lyu
AU  - Toshihiro Obata
AU  - Sanzhen Liu
AU  - Yufeng Ge
AU  - James C. Schnable
AU  - Jinliang Yang
TI  - A historically balanced locus under recent directional selection in responding to changed nitrogen conditions during modern maize breeding
AID  - 10.1101/2022.02.09.479784
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.02.09.479784
4099  - http://biorxiv.org/content/early/2022/02/10/2022.02.09.479784.short
4100  - http://biorxiv.org/content/early/2022/02/10/2022.02.09.479784.full
AB  - Understanding the patterns of selection during plant evolution and recent crop improvement processes is the central topic in plant breeding and genetics. As an essential macronutrient for plant growth and development, nitrogen (N) is a key factor in affecting plant adaptation and crop improvement. The widespread adoption of less expensive industrial N fixation has dramatically reshaped plant morphology by favoring compact maize plants to tolerant crowding stress. The associated genetic changes, however, have not been systematically studied. Here, we investigated maize inbred lines developed before and after the 1960s — the time point when inorganic N fertilizer started to be widely used for maize production. We identified a strong selective sweep exhibiting pronounced genomic differentiation between Old-Era (pre-1960s) and New-Era (post-1960s) inbred lines. Further study revealed population genetics statistics in the sweep exhibited patterns consistent with historical balancing selection. This balanced genomic interval is associated with a number of morphological, physiological, and metabolite traits related to vegetative N responses. A cluster of three glutamate receptor-like (GLR) genes is located within the region targeted by selection. Functional characterizations suggested differences in transcriptional activity of the GLR genes between the haplotypes carried by Old-Era and New-Era inbred lines likely play an essential role in mediating distinct N responses. The identification of both targets of selection and changes in the regulation of N responsive genes between maize lines developed in different eras sheds light on the N sensing and regulation pathways and paves the way to developing N resilient crops.Competing Interest StatementThe authors have declared no competing interest.