RT Journal Article
SR Electronic
T1 Synthetic memory circuits for programmable cell reconfiguration in plants
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.02.11.480167
DO 10.1101/2022.02.11.480167
A1 James P B Lloyd
A1 Florence Ly
A1 Patrick Gong
A1 Jahnvi Pflüger
A1 Tessa Swain
A1 Christian Pflüger
A1 Muhammad Adil Khan
A1 Brendan Kidd
A1 Ryan Lister
YR 2022
UL http://biorxiv.org/content/early/2022/02/12/2022.02.11.480167.abstract
AB Plant biotechnology predominantly relies on a restricted set of genetic parts with limited capability to customize spatiotemporal and conditional expression patterns. Synthetic gene circuits have the ability to integrate multiple customizable input signals through a processing unit constructed from biological parts, to produce a predictable and programmable output. Here, we present a suite of functional recombinase-based gene circuits for use in plants. We first established a range of key gene circuit components compatible with plant cell functionality. We then used these to develop a range of operational logic gates using the identify function (activation) and negation function (repression) in Arabidopsis protoplasts and in vivo, demonstrating their utility for programmable manipulation of transcriptional activity in a complex multicellular organism. Through utilization of genetic recombination these circuits create stable long-term changes in expression and recording of past stimuli. This highly-compact programmable gene circuit platform provides new capabilities for engineering sophisticated transcriptional programs and previously unrealised traits into plants.Competing Interest StatementThe authors have declared no competing interest.