RT Journal Article
SR Electronic
T1 A global lipid map reveals host dependency factors conserved across SARS-CoV-2 variants
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.02.14.480430
DO 10.1101/2022.02.14.480430
A1 Farley, Scotland E.
A1 Kyle, Jennifer E.
A1 Leier, Hans C.
A1 Bramer, Lisa M.
A1 Weinstein, Jules
A1 Bates, Timothy A.
A1 Lee, Joon-Yong
A1 Metz, Thomas O.
A1 Schultz, Carsten
A1 Tafesse, Fikadu G.
YR 2022
UL http://biorxiv.org/content/early/2022/02/15/2022.02.14.480430.abstract
AB A comprehensive understanding of host dependency factors for SARS-CoV-2 remains elusive. We mapped alterations in host lipids following SARS-CoV-2 infection using nontargeted lipidomics. We found that SARS-CoV-2 rewires host lipid metabolism, altering 409 lipid species up to 64-fold relative to controls. We correlated these changes with viral protein activity by transfecting human cells with each viral protein and performing lipidomics. We found that lipid droplet plasticity is a key feature of infection and that viral propagation can be blocked by small-molecule glycerolipid biosynthesis inhibitors. We found that this inhibition was effective against the main variants of concern (alpha, beta, gamma, and delta), indicating that glycerolipid biosynthesis is a conserved host dependency factor that supports this evolving virus.Competing Interest StatementThe authors have declared no competing interest.