RT Journal Article
SR Electronic
T1 Single cell analysis of gene expression in the substantia nigra pars compacta of a pesticide-induced mouse model of Parkinson’s disease
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.02.18.481079
DO 10.1101/2022.02.18.481079
A1 Arshad H. Khan
A1 Lydia K. Lee
A1 Desmond J. Smith
YR 2022
UL http://biorxiv.org/content/early/2022/02/19/2022.02.18.481079.abstract
AB Exposure to pesticides in humans increases the risk of Parkinson’s disease (PD), but the mechanisms remain poorly understood. To elucidate these pathways, we dosed C57BL/6J mice with a combination of the pesticides maneb and paraquat (MNPQ). Behavioral analysis revealed motor deficits consistent with PD. Single cell RNA sequencing of substantia nigra pars compacta revealed both cell-type specific genes and genes expressed differentially between pesticide and control, including Fam241b, Emx2os, Bivm, Gm1439, Prdm15 and Rai2. Neurons had the largest number of significant differentially expressed genes, but comparable numbers were found in astrocytes and less so in oligodendrocytes. In addition, network analysis revealed enrichment in functions related to the extracellular matrix. These findings emphasize the importance of support cells in pesticide-induced PD and refocus our attention away from neurons as the sole agent of this disorder.Competing Interest StatementThe authors have declared no competing interest.