TY - JOUR T1 - Chlamydomonas mutant <em>hpm91</em> lacking PGR5 is a scalable and valuable strain for algal hydrogen (H<sub>2</sub>) production JF - bioRxiv DO - 10.1101/2022.02.23.481610 SP - 2022.02.23.481610 AU - Peng Liu AU - De-Min Ye AU - Mei Chen AU - Jin Zhang AU - Xia-He Huang AU - Li-Li Shen AU - Ke-Ke Xia AU - Xiao-Jing Xu AU - Yong-Chao Xu AU - Ya-Long Guo AU - Ying-Chun Wang AU - Fang Huang Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/02/24/2022.02.23.481610.abstract N2 - Clean and sustainable H2 production is essential toward a carbon-neutral world. H2 generation by Chlamydomonas reinhardtii is an attractive approach for solar-H2 from H2O. However, it is currently not scalable because of lacking ideal strains. Here, we explore hpm91, a previously reported PGR5-deletion mutant with remarkable H2 production, that possesses numerous valuable attributes towards large-scale application and in-depth study issues. We show that hpm91 is at least 100-fold scalable (upto 10 liter) with H2 collection sustained for averagely 26 days and 7287 ml H2/10L-HPBR. Also, hpm91 is robust and active over the period of sulfur-deprived H2 production, most likely due to decreased intracellular ROS relative to wild type. Moreover, quantitative proteomic analysis revealed its features in photosynthetic antenna, primary metabolic pathways and anti-ROS responses. Together with success of new high-H2-production strains derived from hpm91, we highlight that hpm91 is a potent strain toward basic and applied research of algal-H2 photoproduction.Competing Interest StatementThe authors have declared no competing interest. ER -