PT  - JOURNAL ARTICLE
AU  - Beatrice Vione
AU  - Chiara Locatelli
AU  - Giacomo Zavaroni
AU  - Angela Piano
AU  - Giorgia La Rocca
AU  - Maria Caracausi
AU  - Lorenza Vitale
AU  - Allison Piovesan
AU  - Caterina Gori
AU  - Gian Luca Pirazzoli
AU  - Pierluigi Strippoli
AU  - Guido Cocchi
AU  - Giuseppe Ramacieri
AU  - Maria Chiara Pelleri
AU  - Francesca Antonaros
TI  - One-carbon pathway metabolites are altered in the plasma of subjects with Down syndrome: relation to chromosomal dosage
AID  - 10.1101/2021.11.30.470411
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2021.11.30.470411
4099  - http://biorxiv.org/content/early/2022/03/01/2021.11.30.470411.short
4100  - http://biorxiv.org/content/early/2022/03/01/2021.11.30.470411.full
AB  - Down syndrome (DS) is the most common chromosomal disorder and it is caused by trisomy of chromosome 21 (Hsa21). Subjects with DS show a large heterogeneity of phenotypes and the most constant clinical features present are typical facies and intellectual disability (ID). Several studies demonstrated that trisomy 21 causes an alteration in the metabolic profile, involving among all one-carbon cycle. We performed enzyme-linked immunosorbent assays (ELISAs) to identify the concentration of 5 different intermediates of the one-carbon cycle, namely tetrahydrofolate (THF), 5-methyltetrahydrofolate (5-methyl-THF), 5-formyltetrahydrofolate (5-formyl-THF), S-adenosyl-homocysteine (SAH) and S-adenosyl-methionine (SAM) in plasma samples obtained from a total of 164 subjects with DS compared to 54 euploid subjects. Results highlight specific alterations of THF with a median concentration ratio DS/control of 2:3, a decrease of a necessary molecule perfectly consistent with a chromosomal dosage effect. Moreover, SAM and SAH show a ratio of 2:1 and 3.6:1, respectively. The relevance of these results for the biology of intelligence and its impairment in trisomy 21 is discussed, leading to the final proposal of 5-methyl-THF as the best candidate for a clinical trial aimed at restoring the dysregulation of one-carbon cycle in trisomy 21, possibly improving cognitive skills of subjects with DS.Competing Interest StatementThe authors have declared no competing interest.