RT Journal Article SR Electronic T1 A mass spectrometry-based atlas of extracellular matrix proteins across 25 mouse organs JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.03.04.482898 DO 10.1101/2022.03.04.482898 A1 McCabe, Maxwell C. A1 Saviola, Anthony J. A1 Hansen, Kirk C. YR 2022 UL http://biorxiv.org/content/early/2022/03/04/2022.03.04.482898.abstract AB The extracellular matrix is a critical non-cellular component of multi-cellular organisms containing a variety of proteins, glycoproteins, and proteoglycans which has been implicated in a wide variety of essential biological processes, including development, wound healing, and aging. Due to low solubility, many ECM proteins have been underrepresented in previous proteomics datasets. Using an optimized 3-step decellularization and ECM extraction method involving chaotrope extraction and digestion via hydroxylamine hydrochloride, we have generated coverage of the matrisome across 25 organs. We observe that the top 100 most abundant proteins from the ECM fractions of all tissues are generally present in all tissues, indicating that tissue matrices are principally composed of a shared set of ECM proteins. However, these proteins vary up to 4,000-fold between tissues, resulting in highly unique matrix profiles even with the same primary set of proteins. A data reduction approach was used to reveal related networks of expressed ECM proteins across varying tissues, including basement membrane and collagen subtypes.Competing Interest StatementThe authors have declared no competing interest.