PT  - JOURNAL ARTICLE
AU  - Geunhye Hong
AU  - Cadi Davies
AU  - Zahra Omole
AU  - Janie Liaw
AU  - Anna D. Grabowska
AU  - Barbara Canonico
AU  - Nicolae Corcionivoshi
AU  - Brendan Wren
AU  - Nick Dorrell
AU  - Abdi Elmi
AU  - Ozan Gundogdu
TI  - <em>Campylobacter jejuni</em> modulates reactive oxygen species production and NADPH oxidase 1 expression in human intestinal epithelial cells
AID  - 10.1101/2022.03.04.482506
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.03.04.482506
4099  - http://biorxiv.org/content/early/2022/03/04/2022.03.04.482506.short
4100  - http://biorxiv.org/content/early/2022/03/04/2022.03.04.482506.full
AB  - Campylobacter jejuni is the major bacterial cause of foodborne gastroenteritis worldwide. Mechanistically, how this pathogen interacts with intrinsic defence machinery of human intestinal epithelial cells (IECs) remains elusive. To address this, we investigated how C. jejuni counteracts the intracellular and extracellular reactive oxygen species (ROS) in IECs. Our work shows that C. jejuni differentially regulates intracellular and extracellular ROS production in human T84 and Caco-2 cells. C. jejuni downregulates the transcription and translation of Nicotinamide adenine dinucleotide phosphate (NAPDH) oxidase (Nox1), a key ROS-generating enzyme in IECs and antioxidant defence genes cat and sod1. Furthermore, inhibition of Nox1 by diphenylene iodonium (DPI) and siRNA reduced C. jejuni ability to interact, invade and intracellularly survive within T84 and Caco-2 cells. Collectively, these findings provide mechanistic insight into how C. jejuni modulates the IEC defence machinery.Competing Interest StatementThe authors have declared no competing interest.