RT Journal Article
SR Electronic
T1 Comparing lipid remodeling in mouse adipose and liver tissue with quantitative Raman microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.03.07.483297
DO 10.1101/2022.03.07.483297
A1 Alexandra Paul
A1 Belén Chanclón
A1 Cecilia Brännmark
A1 Pernilla Wittung-Stafshede
A1 Charlotta S. Olofsson
A1 Ingrid Wernstedt Asterholm
A1 Sapun H. Parekh
YR 2022
UL http://biorxiv.org/content/early/2022/03/07/2022.03.07.483297.abstract
AB Brown adipose tissue (BAT) consists of highly metabolically active adipocytes that catabolize nutrients to produce heat. Playing an active role in triacylglycerol (TAG) clearance, research has shown that dietary fatty acids can modulate the TAG chemistry deposition in BAT after weeks-long dietary intervention, similar to what has been shown in white adipose tissue (WAT). Our objective was to compare the influence of sustained, non-chronic dietary intervention (a one-week interval) on WAT and BAT lipid metabolism and deposition in situ. We use quantitative, label-free chemical microscopy to show that one week of HFD intervention results in dramatically larger lipid droplet (LD) growth in BAT (and liver) compared to LD growth in inguinal WAT (IWAT). Moreover, BAT showed lipid remodeling as increased unsaturated TAGs in LDs, resembling the dietary lipid composition, while WAT (and liver) did not show lipid remodeling on this time scale. Concurrently, expression of genes involved in lipid metabolism, particularly desaturases, was reduced in BAT and liver from HFD-fed mice after one week. Our data show that BAT lipid chemistry remodels exceptionally fast to dietary lipid intervention compared WAT, which further points towards a role in TAG clearance.Competing Interest StatementThe authors have declared no competing interest.