PT  - JOURNAL ARTICLE
AU  - Linnea Österberg
AU  - Niek Welkenhuysen
AU  - Sebastian Persson
AU  - Stefan Hohmann
AU  - Marija Cvijovic
TI  - Localization and phosphorylation in the Snf1 network is controlled by two independent pathways
AID  - 10.1101/2021.06.14.448401
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2021.06.14.448401
4099  - http://biorxiv.org/content/early/2022/03/10/2021.06.14.448401.short
4100  - http://biorxiv.org/content/early/2022/03/10/2021.06.14.448401.full
AB  - AMPK/SNF1 is the master regulator of energy homeostasis in eukaryotic cells and has a key role in glucose de-repression. If glucose becomes depleted, Snf1 is phosphorylated and activated. Activation of Snf1 is required but is not sufficient for mediating glucose de-repression indicating a second glucose-regulated step that adjusts the Snf1 pathway. To elucidate this regulation, we further explore the spatial dynamics of Snf1 and Mig1 and how they are controlled by concentrations of hexose sugars. We utilize fluorescence recovery after photobleaching (FRAP) to study the movement of Snf1 and how it responds to external glucose concentrations. We show that the Snf1 pathway reacts to the presence of glucose. Furthermore, we identify a negative feedback loop regulating Snf1 activity. Our data offer insight into the true complexity of regulation of this central signaling pathway by one signal (glucose depletion) interpreted by the cell in different ways.Competing Interest StatementThe authors have declared no competing interest.