RT Journal Article SR Electronic T1 Generating minimum set of gRNA to cover multiple targets in multiple genomes with MINORg JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.03.10.481891 DO 10.1101/2022.03.10.481891 A1 Rachelle R.Q. Lee A1 Wei Yuan Cher A1 Eunyoung Chae YR 2022 UL http://biorxiv.org/content/early/2022/03/15/2022.03.10.481891.abstract AB MINORg is an offline gRNA design tool that generates the smallest possible combination of gRNA capable of covering all desired targets in multiple non-reference genomes. As interest in pangenomic research grows, so does the workload required for large screens in multiple individuals. MINORg aims to lessen this workload by capitalising on sequence homology to favour multi-target gRNA while simultaneously screening multiple genetic backgrounds in order to generate reusable gRNA panels. We demonstrated the practical application of MINORg by knocking out a 11 homologous genes tandemly arrayed in a multigene cluster in two Arabidopsis thaliana lineages using three gRNA output by MINORg. Source code is freely available at https://github.com/rlrq/MINORg.Competing Interest StatementThe authors have declared no competing interest.