PT  - JOURNAL ARTICLE
AU  - Brewster, C.E.
AU  - Mann, F.G.
AU  - Benham-Pyle, B.W.
AU  - Sánchez Alvarado, A.
TI  - Syrah, a Slide-seqV2 pipeline augmentation
AID  - 10.1101/2022.03.20.485023
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.03.20.485023
4099  - http://biorxiv.org/content/early/2022/03/21/2022.03.20.485023.short
4100  - http://biorxiv.org/content/early/2022/03/21/2022.03.20.485023.full
AB  - Spatial transcriptomic techniques such as Slide-seqV2 uncover novel relationships and interactions between cell types by coupling gene expression and spatial data. Here we discuss two unexpected sources of error in Slide-seqV2 data, one physical and one computational. To address this we present an analysis pipeline augmentation, Syrah, which corrects for these errors and show that it improves both data quantity and quality over the standard pipeline alone or in combination with additional sequencing.Competing Interest StatementThe authors have declared no competing interest.