RT Journal Article
SR Electronic
T1 Syrah, a Slide-seqV2 pipeline augmentation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.03.20.485023
DO 10.1101/2022.03.20.485023
A1 Brewster, C.E.
A1 Mann, F.G.
A1 Benham-Pyle, B.W.
A1 Sánchez Alvarado, A.
YR 2022
UL http://biorxiv.org/content/early/2022/03/21/2022.03.20.485023.abstract
AB Spatial transcriptomic techniques such as Slide-seqV2 uncover novel relationships and interactions between cell types by coupling gene expression and spatial data. Here we discuss two unexpected sources of error in Slide-seqV2 data, one physical and one computational. To address this we present an analysis pipeline augmentation, Syrah, which corrects for these errors and show that it improves both data quantity and quality over the standard pipeline alone or in combination with additional sequencing.Competing Interest StatementThe authors have declared no competing interest.