RT Journal Article
SR Electronic
T1 Systematic analysis of YFP gene traps reveals common discordance between mRNA and protein across the nervous system
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.03.21.485142
DO 10.1101/2022.03.21.485142
A1 Joshua S Titlow
A1 Maria Kiourlappou
A1 Ana Palanca
A1 Jeffrey Y Lee
A1 Dalia S Gala
A1 Darragh Ennis
A1 Joyce J S Yu
A1 Florence L Young
A1 David Miguel Susano Pinto
A1 Sam Garforth
A1 Helena S Francis
A1 Finn Strivens
A1 Hugh Mulvey
A1 Alex Dallman-Porter
A1 Staci Thornton
A1 Diana Arman
A1 Aino I Järvelin
A1 Mary Kay Thompson
A1 Ilias Kounatidis
A1 Richard M Parton
A1 Stephen Taylor
A1 Ilan Davis
YR 2022
UL http://biorxiv.org/content/early/2022/03/21/2022.03.21.485142.abstract
AB While post-transcriptional control is thought to be required at the periphery of neurons and glia, its extent is unclear. Here, we investigate systematically the spatial distribution and expression of mRNA at single molecule sensitivity and their corresponding proteins of 200 YFP trap protein trap lines across the intact Drosophila nervous system. 98% of the genes studied showed discordance between the distribution of mRNA and the proteins they encode in at least one region of the nervous system. These data suggest that post-transcriptional regulation is very common, helping to explain the complexity of the nervous system. We also discovered that 68.5% of these genes have transcripts present at the periphery of neurons, with 9.5% at the glial periphery. Peripheral transcripts include many potential new regulators of neurons, glia and their interactions. Our approach is applicable to most genes and tissues and includes powerful novel data annotation and visualisation tools for post-transcriptional regulation.Brief outline A novel high resolution and sensitive approach to systematically co-visualise the distribution of mRNAs and proteins in the intact nervous system reveals that post-transcriptional regulation of gene expression is very common. The rich data resource is provided as a browsable resource, which is an exemplar for the characterisation of post-transcriptional regulation of most genes and model systems.Highlights196/200 (98%) Drosophila genes show discordant RNA and protein expression in at least one nervous system region137/200 (68.5%) mRNAs are present in at least one synaptic compartmentNovel localised mRNA and protein discovered in periphery of glial processesNew paradigm for analysis of post-transcriptional regulation and data explorationCompeting Interest StatementI.D. is on the Scientific Advisory Boards of Open Microscopy Environment and Zegami. S.T. is the founder and CSO of Zegami. M.K. is partly funded by Zegami Ltd., acting as an industrial partner for an iCASE studentship. These organisations did not have a role in the study design or interpretation of its findings.