RT Journal Article
SR Electronic
T1 Multicellular PI control for gene regulation in microbial consortia
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.03.21.485171
DO 10.1101/2022.03.21.485171
A1 Vittoria Martinelli
A1 Davide Salzano
A1 Davide Fiore
A1 Mario di Bernardo
YR 2022
UL http://biorxiv.org/content/early/2022/03/22/2022.03.21.485171.abstract
AB We describe two multicellular implementations of the classical P and PI feedback controllers for the regulation of gene expression in a target cell population. Specifically, we propose to distribute the proportional and integral actions over two different cellular populations in a microbial consortium comprising a third target population whose output needs to be regulated. By engineering communication among the different cellular populations via appropriate orthogonal quorum sensing molecules, we are able to close the feedback loop across the consortium. We derive analytical conditions on the biological parameters guaranteeing the regulation of the output of the target population and we validate the robustness and modularity of proposed control schemes via in silico experiments in BSim, a realistic agent-based simulator of bacterial populations.Competing Interest StatementThe authors have declared no competing interest.