RT Journal Article
SR Electronic
T1 3D rotational motion of an endocytic vesicle on a complex microtubule network in a living cell
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 576702
DO 10.1101/576702
A1 S. Lee
A1 H. Higuchi
YR 2019
UL http://biorxiv.org/content/early/2019/03/14/576702.abstract
AB The transport dynamics of endocytic vesicles in a living cell contains essential biomedical information. Although the movement mechanism of a vesicle by motor proteins has been revealed, understanding the precise movement of vesicles on the cytoskeleton in a living cell has been considered challenging, due to the complex 3D network of cytoskeletons. Here, we specify the shape of the 3D interaction between the vesicle and microtubule, based on the theoretically estimated location of the microtubule and the vesicle trajectory data acquired at high spatial and temporal precision. We detected that vesicles showed more frequent direction changes with either in very acute or in obtuse angles than right angles, on similar time scales in a microtubule network. Interestingly, when a vesicle interacted with a relatively longer (> 400 nm) microtubule filament, rotational movement along the axis of the microtubule was frequently observed, implying an obstacle-avoiding motion. Our results are expected to give in-depth insight into understanding the actual 3D interactions between the intracellular molecule and complex cytoskeletal network.