PT  - JOURNAL ARTICLE
AU  - Yang Liu
AU  - Marcello DiStasio
AU  - Graham Su
AU  - Hiromitsu Asashima
AU  - Archibald Enninful
AU  - Xiaoyu Qin
AU  - Yanxiang Deng
AU  - Pino Bordignon
AU  - Marco Cassano
AU  - Mary Tomayko
AU  - Mina Xu
AU  - Stephanie Halene
AU  - Joseph E. Craft
AU  - David Hafler
AU  - Rong Fan
TI  - Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
AID  - 10.1101/2022.04.01.486788
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.04.01.486788
4099  - http://biorxiv.org/content/early/2022/04/02/2022.04.01.486788.short
4100  - http://biorxiv.org/content/early/2022/04/02/2022.04.01.486788.full
AB  - We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.Competing Interest StatementR.F., Y.L., and Y.D. are inventors of a patent application related to this work. R.F. is scientific founder and advisor of IsoPlexis, Singleron Biotechnologies, and AtlasXomics. The interests of R.F. were reviewed and managed by Yale University Provost Office in accordance with the University conflict of interest policies. PB and MC are employees at Lunaphore Technologies SA