RT Journal Article
SR Electronic
T1 Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.04.01.486788
DO 10.1101/2022.04.01.486788
A1 Yang Liu
A1 Marcello DiStasio
A1 Graham Su
A1 Hiromitsu Asashima
A1 Archibald Enninful
A1 Xiaoyu Qin
A1 Yanxiang Deng
A1 Pino Bordignon
A1 Marco Cassano
A1 Mary Tomayko
A1 Mina Xu
A1 Stephanie Halene
A1 Joseph E. Craft
A1 David Hafler
A1 Rong Fan
YR 2022
UL http://biorxiv.org/content/early/2022/04/02/2022.04.01.486788.abstract
AB We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.Competing Interest StatementR.F., Y.L., and Y.D. are inventors of a patent application related to this work. R.F. is scientific founder and advisor of IsoPlexis, Singleron Biotechnologies, and AtlasXomics. The interests of R.F. were reviewed and managed by Yale University Provost Office in accordance with the University conflict of interest policies. PB and MC are employees at Lunaphore Technologies SA