TY - JOUR T1 - In Utero Activation of NK Cells in Congenital CMV Infection JF - bioRxiv DO - 10.1101/2022.04.04.487059 SP - 2022.04.04.487059 AU - Anna V Vaaben AU - Justine Levan AU - Catherine B T Nguyen AU - Perri C Callaway AU - Mary Prahl AU - Lakshmi Warrier AU - Felistas Nankya AU - Kenneth Musinguzi AU - Abel Kakuru AU - Mary K Muhindo AU - Grant Dorsey AU - Moses R. Kamya AU - Margaret E. Feeney Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/04/05/2022.04.04.487059.abstract N2 - Background Congenital cytomegalovirus (CMV) infection is the most common infectious cause of birth defects and neurological damage in newborns. Despite a well-established role for NK cells in control of CMV infection in older children and adults, it remains unknown whether fetal NK cells can sense and respond to CMV infection acquired in utero.Methods Here, we investigate the impact of congenital CMV infection on the neonatal NK cell repertoire by assessing the frequency, phenotype, and functional profile of NK cells in cord blood samples from newborns with congenital CMV and from uninfected controls enrolled in a birth cohort of Ugandan mothers and infants.Results We find that neonatal NK cells from congenitally CMV infected newborns show increased expression of cytotoxic mediators, signs of maturation and activation, and an expansion of mature CD56-negative NK cells, an NK cell subset associated with chronic viral infections in adults. Activation was particularly prominent in NK cell subsets expressing the Fcγ receptor CD16, indicating a role for antibody-mediated immunity against CMV in utero.Conclusion These findings demonstrate that NK cells can be activated in utero and suggest that NK cells may be an important component of the fetal and infant immune response against CMV.Competing Interest StatementThe authors have declared no competing interest. ER -