PT  - JOURNAL ARTICLE
AU  - H. Abramczyk
AU  - M. Kopeć
AU  - K. Beton
TI  - Hyperglycemia and Cancer. Human lung carcinoma by means of Raman spectroscopy and imaging
AID  - 10.1101/2022.04.05.487128
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.04.05.487128
4099  - http://biorxiv.org/content/early/2022/04/07/2022.04.05.487128.short
4100  - http://biorxiv.org/content/early/2022/04/07/2022.04.05.487128.full
AB  - Raman spectroscopy and Raman imaging allow to identify the biochemical and structural features of human cancer lung cell line (CCL-185) and the cell line supplemented with glucose and deuterated glucose in normal and hyperglycemia conditions. We found that isotope substitution of glucose by deuterated glucose allows to separate de novo lipid synthesis from exogenous uptake of lipids obtained from the diet. We demonstrated that glucose is largely utilized for de novo lipid synthesis. Our results provide a direct evidence that high level of glucose decreases the metabolism via oxidative phosporylation in mitochondria in cancer cells and shifts the metabolism to glycolysis via Wartburg effect.Competing Interest StatementThe authors have declared no competing interest.