RT Journal Article
SR Electronic
T1 Hyperglycemia and Cancer. Human lung carcinoma by means of Raman spectroscopy and imaging
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.04.05.487128
DO 10.1101/2022.04.05.487128
A1 H. Abramczyk
A1 M. Kopeć
A1 K. Beton
YR 2022
UL http://biorxiv.org/content/early/2022/04/07/2022.04.05.487128.abstract
AB Raman spectroscopy and Raman imaging allow to identify the biochemical and structural features of human cancer lung cell line (CCL-185) and the cell line supplemented with glucose and deuterated glucose in normal and hyperglycemia conditions. We found that isotope substitution of glucose by deuterated glucose allows to separate de novo lipid synthesis from exogenous uptake of lipids obtained from the diet. We demonstrated that glucose is largely utilized for de novo lipid synthesis. Our results provide a direct evidence that high level of glucose decreases the metabolism via oxidative phosporylation in mitochondria in cancer cells and shifts the metabolism to glycolysis via Wartburg effect.Competing Interest StatementThe authors have declared no competing interest.