RT Journal Article
SR Electronic
T1 LILAC: Enhanced actin imaging with an optogenetic Lifeact
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.04.07.487416
DO 10.1101/2022.04.07.487416
A1 Kroll, Kourtney L.
A1 French, Alexander R.
A1 Sosnick, Tobin R.
A1 Rock, Ronald S.
YR 2022
UL http://biorxiv.org/content/early/2022/04/08/2022.04.07.487416.abstract
AB We have designed an improved Lifeact variant that binds to actin under the control of light using the LOV2 protein. Light control enables one to subtract the pre-illumination signal of the unbound label, yielding an enhanced view of F-actin dynamics in cells. Furthermore, the tool eliminates actin network perturbations and cell sickness caused by Lifeact overexpression.Competing Interest StatementA provisional patent of the methods included in the manuscript are included in a provisional patent filing #63253420 entitled "Methods and Compositions Comprising Actin Binding Proteins".