RT Journal Article
SR Electronic
T1 Spatially resolved gene regulatory and disease vulnerability map of the adult Macaque cortex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.14.087601
DO 10.1101/2020.05.14.087601
A1 Ying Lei
A1 Mengnan Cheng
A1 Zihao Li
A1 Zhenkun Zhuang
A1 Liang Wu
A1 Yunong Sun
A1 Lei Han
A1 Zhihao Huang
A1 Yuzhou Wang
A1 Zifei Wang
A1 Liqin Xu
A1 Yue Yuan
A1 Shang Liu
A1 Taotao Pan
A1 Jiarui Xie
A1 Chuanyu Liu
A1 Giacomo Volpe
A1 Carl Ward
A1 Yiwei Lai
A1 Jiangshan Xu
A1 Mingyue Wang
A1 Hao Yu
A1 Haixi Sun
A1 Qichao Yu
A1 Liang Wu
A1 Chunqing Wang
A1 Chi Wai Wong
A1 Wei Liu
A1 Liangzhi Xu
A1 Jingkuan Wei
A1 Dandan Chen
A1 Zhouchun Shang
A1 Guibo Li
A1 Kun Ma
A1 Le Cheng
A1 Fei Ling
A1 Tao Tan
A1 Kai Chen
A1 Bosiljka Tasic
A1 Michael Dean
A1 Weizhi Ji
A1 Huanming Yang
A1 Ying Gu
A1 Miguel A. Esteban
A1 Yuxiang Li
A1 Ao Chen
A1 Yuyu Niu
A1 Hongkui Zeng
A1 Yong Hou
A1 Longqi Liu
A1 Shiping Liu
A1 Xun Xu
YR 2022
UL http://biorxiv.org/content/early/2022/04/11/2020.05.14.087601.abstract
AB Single cell approaches have increased our knowledge about the cell type composition of the non-human primate (NHP), but a detailed characterization of area-specific regulatory features remains outstanding. We generated single-cell chromatin accessibility (single-cell ATAC) and transcriptomic data of 358,237 cells from prefrontal cortex (PFC), primary motor cortex (M1) and primary visual cortex (V1) of adult cynomolgus monkey brain, and integrated this dataset with Stereo-seq (Spatio-Temporal Enhanced REsolution Omics-sequencing) of the corresponding cortical areas to assign topographic information to molecular states. We identified area-specific chromatin accessible sites and their targeted genes, including the cell type-specific transcriptional regulatory network associated with excitatory neurons heterogeneity. We reveal calcium ion transport and axon guidance genes related to specialized functions of PFC and M1, identified the similarities and differences between adult macaque and human oligodendrocyte trajectories, and mapped the genetic variants and gene perturbations of human diseases to NHP cortical cells. This resource establishes a transcriptomic and chromatin accessibility combinatory regulatory landscape at a single-cell and spatially resolved resolution in NHP cortex.Competing Interest StatementThe authors have declared no competing interest.