RT Journal Article
SR Electronic
T1 Berberine Bridge Enzyme-Like Oligosaccharide Oxidases Act As Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant Peroxidases
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.04.15.488465
DO 10.1101/2022.04.15.488465
A1 Anna Scortica
A1 Moira Giovannoni
A1 Valentina Scafati
A1 Francesco Angelucci
A1 Felice Cervone
A1 Giulia De Lorenzo
A1 Manuel Benedetti
A1 Benedetta Mattei
YR 2022
UL http://biorxiv.org/content/early/2022/04/15/2022.04.15.488465.abstract
AB OG-oxidases (OGOXs) and CD-oxidase (CELLOX) are plant berberine bridge enzyme-like oligosaccharide oxidases that oxidize oligogalacturonides (OGs) and cellodextrins (CDs), cell wall fragments with nature of damage-associated molecular patterns (DAMPs). The oxidation of OGs and CDs attenuates their elicitor activity by concomitantly releasing H2O2. Here, we demonstrate that the H2O2 generated downstream of the combined action between a fungal polygalacturonase and OGOX1 or an endoglucanase and CELLOX can be directed by plant peroxidases (PODs) either towards a reaction possibly involved in plant defence such as the oxidation of monolignol or a reaction possibly involved in a developmental event such as the oxidation of auxin (IAA), pointing to OGOX1 and CELLOX as enzymatic transducers between microbial glycoside hydrolases and plant PODs.Competing Interest StatementThe authors have declared no competing interest.ABTS2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)AnEGendoglucanase from Aspergillus niger (poly-α-1,4-galacturonide glycanohydrolase, EC number: 3.2.1.15)APODanionic peroxidase from ripe tomato fruit (phenolic donor: hydrogen-peroxide oxidoreductase, EC number: 1.11.1.7)BBEBerberine Bridge Enzyme [(S)-reticuline:oxygen oxidoreductase, EC number: 1.21.3.3]BBE-lBerberine Bridge Enzyme-likeCATcatalase from bovine liver (hydrogen-peroxide:hydrogen-peroxide oxidoreductase, EC number: 1.11.1.6)CDcellodextrinCELLOXcellodextrin-oxidase, CD-oxidase (cellodextrin:oxygen oxidoreductase, EC number: 1.21.3.3)DAMPdamage-associated molecular patternGHglycoside hydrolaseHRPhorseradish peroxidase VI-A type (phenolic donor: hydrogen-peroxide oxidoreductase, EC number: 1.11.1.7)IAAindole-3-acetic acid, auxinOGoligogalacturonideOGOXoligogalacturonide-oxidase, OG-oxidase (oligogalacturonide:oxygen oxidoreductase, EC number: 1.21.3.3)FpPGendopolygalacturonase from Fusarium phyllophilum (poly-α-1,4-galacturonide glycanohydrolase, EC number: 3.2.1.15)PODperoxidase (phenolic donor: hydrogen-peroxide oxidoreductase, EC number: 1.11.1.7)