RT Journal Article
SR Electronic
T1 Binding of the peptide deformylase on the ribosome surface modulates the structure and dynamics of the exit tunnel interior
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.04.20.488877
DO 10.1101/2022.04.20.488877
A1 Hugo McGrath
A1 Michaela Černeková
A1 Michal H. Kolář
YR 2022
UL http://biorxiv.org/content/early/2022/04/20/2022.04.20.488877.abstract
AB Proteosynthesis on ribosomes is regulated at many levels. Conformational changes of the ribosome, possibly induced by external factors, may transfer over large distances and contribute to the regulation. The principles of this long-distance allostery within the ribosome remain poorly understood. Here, we use structural analysis and atomistic molecular dynamics simulations to investigate peptide deformylase (PDF), an enzyme that binds to the ribosome surface near the ribosomal protein uL22 during translation and chemically modifies the emerging nascent peptide. Our simulations of the entire ribosome–PDF complex reveal that the PDF undergoes a swaying motion on the ribosome surface at the sub-microsecond time scale. We show that the PDF affects the conformational dynamics of parts of the ribosome over distances of more than 5 nm. Using a supervised-learning algorithm we demonstrate that the exit tunnel is influenced by the presence or absence of PDF. Our findings suggest a possible effect of the PDF on the nascent peptide translocation through the ribosome exit tunnel.Competing Interest StatementThe authors have declared no competing interest.