RT Journal Article
SR Electronic
T1 Integrated optical device for Structured Illumination Microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.04.22.489094
DO 10.1101/2022.04.22.489094
A1 Matteo Calvarese
A1 Petra Paiè
A1 Alessia Candeo
A1 Gianmaria Calisesi
A1 Francesco Ceccarelli
A1 Gianluca Valentini
A1 Roberto Osellame
A1 Hai Gong
A1 Mark A. Neil
A1 Francesca Bragheri
A1 Andrea Bassi
YR 2022
UL http://biorxiv.org/content/early/2022/04/22/2022.04.22.489094.abstract
AB Structured Illumination Microscopy (SIM) is a key technology for high resolution and super-resolution imaging of biological cells and molecules. The spread of portable and easy-to-align SIM systems requires the development of novel methods to generate a light pattern and to shift it across the field of view of the microscope. Here we show a miniaturized chip that incorporates optical waveguides, splitters, and phase shifters, to generate a 2D structured illumination pattern suitable for SIM microscopy. The chip creates three point-sources, coherent and controlled in phase, without the need for further alignment. Placed in the pupil of a microscope’s objective, the three sources generate a hexagonal illumination pattern on the sample, which is spatially translated thanks to thermal phase shifters. We validate and use the chip, upgrading a commercial inverted fluorescence microscope to a SIM setup and we image biological sample slides, extending the resolution of the microscope.Competing Interest StatementThe authors have declared no competing interest.