TY - JOUR T1 - High-resolution line-scan Brillouin microscopy for live-imaging of mechanical properties during embryo development JF - bioRxiv DO - 10.1101/2022.04.25.489364 SP - 2022.04.25.489364 AU - Carlo Bevilacqua AU - Juan Manuel Gomez AU - Ulla-Maj Fiuza AU - Chii Jou Chan AU - Ling Wang AU - Sebastian Hambura AU - Manuel Eguren AU - Jan Ellenberg AU - Alba Diz-Muñoz AU - Maria Leptin AU - Robert Prevedel Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/04/26/2022.04.25.489364.abstract N2 - Brillouin microscopy (BM) can be used to assess the mechanical properties of biological samples in a 3D, all-optical, and hence non-contact fashion, but its weak signals require long imaging times and illumination dosages harmful to living organisms. Here, we present a line-scanning Brillouin microscope optimized for fast and high-resolution live-imaging of dynamic biological processes with low photo-toxicity. In combination with fluorescence light-sheet imaging, we demonstrate the capabilities of our microscope to visualize the mechanical properties of cells and tissues over space and time in living model organisms such as fruit flies, ascidians, and mouse embryos.Competing Interest StatementJ. Ellenberg is scientific co-founder and advisor of Luxendo, part of Bruker, which makes light-sheet-based microscopes commercially available. The other authors declare no competing financial interests. ER -