PT - JOURNAL ARTICLE AU - Yen Thi Ngoc Nguyen AU - Chung Dinh Duong AU - Hong Thi Kim Nguyen AU - Cham Phuong Nguyen AU - Nhan Thi Vo AU - Linh Thi Lan Dinh AU - Ngoc Le Bao Nguyen AU - Thai Minh Nguyen AU - Nga Dinh Nguyen AU - Anh Tu Nguyen TI - MYXOBACTERIA FROM VIETNAM: ISOLATION, PHYLOGENETIC ANALYSIS AND BIOPROSPECTION AID - 10.1101/2022.04.27.489816 DP - 2022 Jan 01 TA - bioRxiv PG - 2022.04.27.489816 4099 - http://biorxiv.org/content/early/2022/04/28/2022.04.27.489816.short 4100 - http://biorxiv.org/content/early/2022/04/28/2022.04.27.489816.full AB - Forty-three myxobacterial strains were isolated and identified from 20 soil samples collected in Vietnam. The information on morphological characteristics and 16S ribosomal gene sequencing showed that these strains were designated to seven genera belonging to Angiococcus, Archangium, Chondromyces, Corallococcus, Cystobacter, Melittangium, and Myxococcus, in suborder Cystobacterineae and Sorangiineae. The phylogenetic tree was constructed to clarify the genetic relationship between myxobacterial isolates. Myxobacteria were cultured, and crude extracts were obtained after 10-day fermentation in P-medium in the presence of the Amberlite XAD 16N adsorbent resin. Elution was carried out with acetone and methanol to obtain the crude extracts. Evaluation of antioxidant activity used the DPPH and ABTS assay, the minimum inhibitory concentration values were determined by the microdilution method. The total extract from CT21 had the highest total antioxidant activity (IC50 = 52.34 ± 1.47 µg/mL, 30.28 ± 0.74 µg/mL for DPPH and ABTS assays, respectively). The other potential strain was TG131 and GL41 that IC50 values were 40.28 ± 1.13 and 57.24 ± 1.52 µg/mL, respectively (by the DPPH method), and 48.35 ± 0.58 and 42.76 ± 0.50 µg/mL, respectively (by the ABTS method). Interestingly, 100% isolated myxobacterial strains show inhibitory activity against at least one of the tested microorganisms. The potential antimicrobial strain was GL41, which inhibited all tested microorganisms, and the MIC values were 1 µg/mL against MRSA, MSSA, S. faecalis, C. albicans, and A. niger. The highest active strains were members of Myxococcus sp. genus.Competing Interest StatementThe authors have declared no competing interest.