RT Journal Article
SR Electronic
T1 Content-aware image restoration improves spatiotemporal resolution in luminescence microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.04.29.490012
DO 10.1101/2022.04.29.490012
A1 Boothe, Tobias
A1 Ivanković, Mario
A1 Grohme, Markus A.
A1 Rink, Jochen C.
YR 2022
UL http://biorxiv.org/content/early/2022/04/29/2022.04.29.490012.abstract
AB Luminescence microscopy is a powerful tool in biomedical imaging applications due to its intrinsically high signal to noise ratio. However, luminescence signal detection requires longer exposure times than fluorescence imaging and is consequently less suited for applications requiring high temporal resolution or throughput. Here we demonstrate that content-aware image restoration can drastically reduce the exposure time requirements in luminescence imaging, thus overcoming one of the major limitations of the technique.Competing Interest StatementThe authors have declared no competing interest.