PT  - JOURNAL ARTICLE
AU  - Deniz Saltukoglu
AU  - Bugra Özdemir
AU  - Michael Holtmannspötter
AU  - Ralf Reski
AU  - Jacob Piehler
AU  - Rainer Kurre
AU  - Michael Reth
TI  - Plasma membrane topography governs the three-dimensional dynamic localization of IgM B cell receptor clusters
AID  - 10.1101/2022.04.29.489661
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.04.29.489661
4099  - http://biorxiv.org/content/early/2022/04/29/2022.04.29.489661.short
4100  - http://biorxiv.org/content/early/2022/04/29/2022.04.29.489661.full
AB  - B lymphocytes recognize bacterial or viral antigens via different classes of the B cell antigen receptor (BCR). Protrusive structures termed microvilli cover lymphocyte surfaces and are thought to perform sensory functions in screening antigen-bearing surfaces. Here, we have studied the cell surface features of Ramos B cells and the spatiotemporal organization of the IgM-BCR using lattice light sheet microscopy in combination with tailored custom-built 4D image analysis. Ramos B cell surfaces were found to form dynamic networks of elevated ridges bridging individual microvilli. A proportion of membrane-localized IgM-BCR was found in clusters, which were associated with the ridges and the microvilli. The dynamic ridge network organization and the IgM-BCR cluster mobility were linked and both were controlled by Arp2/3 complex activity. Our results suggest that topographical features of the cell surface govern the distribution and dynamic localization of IgM-BCR clusters to facilitate antigen screening.Competing Interest StatementThe authors have declared no competing interest.