RT Journal Article
SR Electronic
T1 Actuating tension-loaded DNA clamps drives membrane tubulation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.05.02.490361
DO 10.1101/2022.05.02.490361
A1 Longfei Liu
A1 Qiancheng Xiong
A1 Chun Xie
A1 Frederic Pincet
A1 Chenxiang Lin
YR 2022
UL http://biorxiv.org/content/early/2022/05/03/2022.05.02.490361.abstract
AB Membrane dynamics in living organisms can arise from proteins adhering to, assembling on, and exerting force on cell membranes. Programmable synthetic materials, such as self-assembled DNA nanostructures, offer the capability to drive membrane remodeling events in a way that resembles protein-mediated dynamics, but with user-defined outcomes. An example showcasing this capability is the tubular deformation of liposomes by DNA nanostructures with purposely designed shapes, surface modifications, and self-assembling properties. However, stimulus-responsive membrane tubulation mediated by DNA structure reconfiguration remains challenging. Here we present the triggered formation of membrane tubes in response to specific DNA signals that actuate membrane-bound DNA clamps from an open state to various predefined closed states, releasing pre-stored energy to activate membrane deformation. Using giant unilamellar vesicles (GUVs) as a model system, we show that the timing and efficiency of tubulation, as well as the width of membrane tubes, are modulated by the conformational change of DNA clamps, marking a solid step toward spatiotemporal control of membrane dynamics in an artificial system.Competing Interest StatementThe authors have declared no competing interest.