@article {Li2022.05.05.490809, author = {Bingnan Li and Patrice Zeis and Alisa Alekseenko and Gen Lin and Manu M Tekkedil and Lars M. Steinmetz and Vicent Pelechano}, title = {Differential regulation of mRNA stability modulates transcriptional memory and facilitates environmental adaptation}, elocation-id = {2022.05.05.490809}, year = {2022}, doi = {10.1101/2022.05.05.490809}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Transcriptional memory, by which cells respond faster to repeated stimuli, is key for cellular adaptation and organism survival. Factors related to chromatin organization and activation of transcription have been shown to play a role in the faster response of those cells previously exposed to a stimulus (primed). However, the contribution of post-transcriptional regulation is not yet explored. Here, combining flow cytometry and high throughput sequencing, we perform a genome-wide screen to identify novel factors modulating transcriptional memory in S. cerevisiae in response to galactose nutrition sources. In addition to the well-known chromatin factors modulating transcriptional memory, we find that depletion of the nuclear RNA exosome increases GAL1 expression in primed cells. We perform a genome-wide characterisation of this process and show that changes in nuclear surveillance factor association can enhance both gene induction and repression in primed cells. Finally, we show that in addition to nuclear mRNA degradation, differences in cytoplasmic mRNA decay also modulate transcriptional memory and contribute to faster gene expression remodelling in primed cells. Our results demonstrate that mRNA post-transcriptional regulation, and not only transcription regulation, should be considered when investigating gene expression memory.Competing Interest StatementThe authors have declared no competing interest.}, URL = {https://www.biorxiv.org/content/early/2022/05/05/2022.05.05.490809}, eprint = {https://www.biorxiv.org/content/early/2022/05/05/2022.05.05.490809.full.pdf}, journal = {bioRxiv} }