PT  - JOURNAL ARTICLE
AU  - Tsolaki, Elena
AU  - Chester, Adrian H
AU  - Bertazzo, Sergio
TI  - Density-Dependent Color Scanning Electron Microscopy (DDC-SEM) for calcified tissue and pathological calcification
AID  - 10.1101/2022.05.06.490880
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.05.06.490880
4099  - http://biorxiv.org/content/early/2022/05/06/2022.05.06.490880.short
4100  - http://biorxiv.org/content/early/2022/05/06/2022.05.06.490880.full
AB  - Scanning electron microscopy (SEM) is widely used for materials characterization. It has also been successfully applied to the imaging of biological samples, providing invaluable insights into the topography, morphology and composition of biological structures, including pathological minerals, in diseases affecting cardiovascular, kidney and ocular tissues. Here we provide a comprehensive and detailed guide on how to use colored SEM to aid the visualization and characterization of pathological calcification, and identify the effects of different sample preparation protocols for the visualisation of these minerals.Competing Interest StatementThe authors have declared no competing interest.