PT  - JOURNAL ARTICLE
AU  - Axel Ekman
AU  - Jian-Hua Chen
AU  - Bieke Vanslembrouck
AU  - Carolyn A Larabell
AU  - Mark A Le Gros
AU  - Venera Weinhardt
TI  - Extending of imaging volume in soft x-ray tomography
AID  - 10.1101/2022.05.11.491437
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.05.11.491437
4099  - http://biorxiv.org/content/early/2022/05/11/2022.05.11.491437.short
4100  - http://biorxiv.org/content/early/2022/05/11/2022.05.11.491437.full
AB  - Soft x-ray tomography offers rapid whole single cell imaging with a few tens of nanometers spatial resolution without fixation or labelling. At the moment, this technique is limited to 10 µm thick specimens, such that applications of soft x-ray tomography to large human cells or multicellular specimens are not possible. We have developed a theoretical and experimental framework for soft x-ray tomography to enable extension of imaging volume to 18 µm thick specimens. This approach, based on long depth of field and half-acquisition tomography, is easily applicable to existing full-rotation based microscopes. This opens applications for imaging of large human cells, which are often observed in cancer research and cell to cell interactions.Competing Interest StatementThe authors have declared no competing interest.