RT Journal Article
SR Electronic
T1 Extending of imaging volume in soft x-ray tomography
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.05.11.491437
DO 10.1101/2022.05.11.491437
A1 Axel Ekman
A1 Jian-Hua Chen
A1 Bieke Vanslembrouck
A1 Carolyn A Larabell
A1 Mark A Le Gros
A1 Venera Weinhardt
YR 2022
UL http://biorxiv.org/content/early/2022/05/11/2022.05.11.491437.abstract
AB Soft x-ray tomography offers rapid whole single cell imaging with a few tens of nanometers spatial resolution without fixation or labelling. At the moment, this technique is limited to 10 µm thick specimens, such that applications of soft x-ray tomography to large human cells or multicellular specimens are not possible. We have developed a theoretical and experimental framework for soft x-ray tomography to enable extension of imaging volume to 18 µm thick specimens. This approach, based on long depth of field and half-acquisition tomography, is easily applicable to existing full-rotation based microscopes. This opens applications for imaging of large human cells, which are often observed in cancer research and cell to cell interactions.Competing Interest StatementThe authors have declared no competing interest.