TY - JOUR T1 - Adding a new dimension: Multi-level structure and organization of mixed-species <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> biofilms in a 4-D wound microenvironment JF - bioRxiv DO - 10.1101/2022.05.14.491929 SP - 2022.05.14.491929 AU - Radhika Dhekane AU - Shreeya Mhade AU - Karishma S Kaushik Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/05/14/2022.05.14.491929.abstract N2 - Biofilms in wounds typically consist of aggregates of bacteria, most often Pseudomonas aeruginosa and Staphylococcus aureus, in close association with each other and the host microenvironment. Given this, the interplay across host and microbial elements, including the biochemical and nutrient profile of the microenvironment, likely influences the structure and organization of wound biofilms. While clinical studies, in vivo and ex vivo model systems have provided insights into the distribution of P. aeruginosa and S. aureus in wounds, they are limited in their ability to provide a detailed characterization of biofilm structure and organization across the host-microbial interface. On the other hand, biomimetic in vitro systems, such as host cell surfaces and simulant media conditions, albeit reductionist, have been shown to support the co-existence of P. aeruginosa and S. aureus biofilms, with species-dependent localization patterns and interspecies interactions. Therefore, composite in vitro models that bring together key features of the wound microenvironment could provide unprecedented insights into the structure and organization of mixed-species biofilms. We have built a four-dimensional (4-D) wound microenvironment consisting of a 3-D host cell scaffold of co-cultured human epidermal keratinocytes and dermal fibroblasts, and an in vitro wound milieu (IVWM); the IVWM provides the fourth dimension that represents the biochemical and nutrient profile of the wound infection state. We leveraged this composite 4-D wound microenvironment to probe the structure of mixed-species P. aeruginosa and S. aureus biofilms across multiple levels of organization such as aggregate dimensions and biomass thickness, species co-localization and organization within the biomass, overall biomass composition and interspecies interactions. In doing so, the composite 4-D wound microenvironment platform provides multi-level insights into the structure of mixed-species biofilms, which we incorporate into the current understanding of P. aeruginosa and S. aureus organization in the wound bed.Competing Interest StatementThe authors have declared no competing interest. ER -