RT Journal Article SR Electronic T1 Biocompatible polymers for scalable production of human neural organoids JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.03.18.484949 DO 10.1101/2022.03.18.484949 A1 Narazaki, Genta A1 Miura, Yuki A1 Pavlov, Sergey D. A1 Thete, Mayuri Vijay A1 Roth, Julien G. A1 Shin, Sungchul A1 Heilshorn, Sarah C. A1 Pașca, Sergiu P. YR 2022 UL http://biorxiv.org/content/early/2022/05/17/2022.03.18.484949.abstract AB The generation of neural organoids from human pluripotent stem cells holds great promise in modeling disease and screenings drugs, but current approaches are difficult to scale due to undesired organoid fusion. Here, we develop a scalable neural organoid platform by screening biocompatible polymers that prevent fusion of organoids cultured in suspension. We show that addition of one inexpensive polysaccharide enables straightforward screening of 298 FDA-approved drugs and teratogens for growth defects using over 2,400 cortical organoids.Competing Interest StatementG.N. was an employee of Daiichi-Sankyo Co., Ltd, during the duration of this study, but the company did not have any input on the design of experiments and interpretation of the data. Stanford University holds a patent that covers the generation of cortical organoids (US patent 62/477,858), which has been commercially licensed to STEMCELL Technologies. S.P.P. is listed as an inventor on this patent. All other authors declare no competing interests.