RT Journal Article
SR Electronic
T1 Breathing dysfunction and alveolar damage in a mouse model of Dravet syndrome
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.05.20.492889
DO 10.1101/2022.05.20.492889
A1 Min-Jee Goh
A1 Cassandra E. Deering-Rice
A1 Jacklyn Nguyen
A1 Evalien Duyvesteyn
A1 Alessandro Venosa
A1 Christopher A. Reilly
A1 Cameron S. Metcalf
YR 2022
UL http://biorxiv.org/content/early/2022/05/21/2022.05.20.492889.abstract
AB Objective The incidence of Sudden Unexpected Death in Epilepsy (SUDEP) is especially high in those with Dravet syndrome (DS). Risk factors have been identified, but the mechanism(s) by which death occurs is not fully understood. Evidence supports ventilatory dysfunction in the pathophysiology of SUDEP. Understanding specific respiratory patterns present at baseline and after seizures at different ages, as well as the health of lung tissue, will allow us to better understand how sudden death occurs in this population.Methods Whole body plethysmography (WBP) was used to monitor respiration before and after electrically induced seizure in the Scn1aA1783V/WT mouse model of DS weekly for a period of four weeks. Following the four-week WBP study, lungs from surviving animals were collected and stained with hematoxylin and eosin and Weigert’s elastin and the density of tissue and elastin were analyzed.Results Breathing was diminished in the DS mouse at baseline and following evoked seizures in younger aged mice (P18-P24), consistent with prolonged post-ictal inspiratory time and low respiratory drive compared to the response seen in older animals. In older DS mice, consisting of those that have survived a critical period for mortality, the response to seizure was more robust and included higher respiratory drive, peak inspiratory and expiratory flow rates, tidal and expiratory volumes, and breathing frequency compared to wild-type and relative to baseline. Alveolar damage was also observed in P46-P52 DS mice.Significance Differences in specific respiratory parameters in younger DS animals, during the time when mortality is greatest, compared to older DS animals (i.e. those that have survived the critical period) may allow us to better understand respiratory differences contributing to SUDEP. Lung tissue damage in DS may also contribute to respiratory dysfunction in SUDEP.KEY POINTSBaseline respiration is diminished in DS mice compared to wild type.Electrically induced seizure produced a different respiratory response in younger DS mouse compared to older DS animals.Alveolar septal damage is present in DS mice.Baseline and post-ictal breathing dysfunction and inefficient oxygenation and CO2 clearance likely potentiated by lung damage may serve as a potential mechanism by which SUDEP occurs in DS.Competing Interest StatementThe authors have declared no competing interest.